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Transcriptome+Metabolome

The transcriptome is an important method to obtain gene expression in a biological system, and the metabolome is the basis and direct readout of the system’s phenotype. The metabolites are the final result of gene transcription under internal and external regulation and are the material basis of the observed phenotype. In the era of systems biology research, biological processes and gene regulatory networks are complex and dynamic. It is often insufficient to use a single dataset to study systems biology. Correlating transcriptomic data that has a large number of differentially expressed genes with differential metabolites detected by metabolomics can pinpoint key genes, metabolites, and metabolic pathways that are closely associated with internal changes in the system, and thereby explain biological problems in a more holistic approach.

Technology Introduction of Transcriptome+Metabolome Services

Correlating transcriptomic data that has a large number of differentially expressed genes with differential metabolites detected by metabolomics can pinpoint key genes, metabolites, and metabolic pathways that are closely associated with internal changes in the system, and thereby explain biological problems in a more holistic approach.

Transcriptome+Metabolome Services Case Study

The Cardamine Enshiensis Genome Reveals Whole Genome Duplication And Insight Into Selenium Hyperaccumulation And Tolerance (Huang Et Al., Cell Discovery 2021)
Project Workflow of Transcriptome+Metabolome Services

1

Sample Requirements of Transcriptome+Metabolome Services Service

Sample Type

Minimum requirement per sample

Storage and transportation

Whole blood

2ml

For LCMS

Snap freeze in liquid nitrogen.

Store in -80C.

Ship with dry ice.


For RNAseq

Prepare whole blood with 3x Trizol

Resuspend cells with 1ml TRIzol for every 5x10 6 cells.

Snap freeze materials in liquid nitrogen and store all materials in -80C

Tissue

300mg

Cultured Cells

1 x 10 7 cells

Plant materials

3g

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WHAT'S NEXT IN OMICS: THE METABOLOME

Please submit a detailed description of your project. We will provide you with a customized project plan metabolomics services to meet your research requests. You can also send emails directly to support-global@metwarebio.com for inquiries.
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