Quantitative Lipidomics
Quantitative Lipidomics
Technology Introduction
Technical Features of Quantitative Lipidomics Service
Applications of Quantitative Lipidomics Service
- Clinical disease research: applied to various types of disease research such as cardiovascular, tumor, immune, endocrine, gastrointestinal, and neurological.
- Screening of Biomarkers: by studying the differences in lipid profiles between different groups, screening of Biomarkers can be used for research on disease diagnosis, disease staging/staging, prognosis determination, etc.
- Mechanism studies: e.g. mechanism studies of lipid effects on growth, development, energy metabolism, signal transduction processes, and diseases.
- Pharmaceutical therapy: e.g. polyketide anti-microbial, anti-parasitic and anti-tumor drugs.
- Multi-omics joint research: combined with other histologies to study the physiological and pathological effects of the organism, such as the elucidation of regulatory genes of important lipids that affect disease health and the mechanisms of regulation.
Demo Final Report Of Quantitative Lipidomics
Quantitative Lipidomics Service Case Study
| Number of Lipids | ||
| Class I | Class II | Number |
| Fatty acyls(FA) | CAR, FFA, Eicosanoid, FAHFA | 270 |
| Glycerolipids(GL) | DG, DG-O, MG, TG, TG-O, MGDG, DGDG | 1015 |
| Glycerophospholipids(GP) | LPC, LPC-O, LPE, LPE-P, LPG, LPS, PC, PC-O, PE, PE-P, PE-O, PG, PS, LPI, PI, LPA, PA, PMeOH, BMP, HMBP, LNAPE | 1800 |
| Sphingolipids(SL) | SPH, CerP, HexCer, SM, Cer, Cert | 828 |
| Sterol lipids(ST) | Cho, CE, BA, CASE | 122 |
| Prenol lipids(PR) | CoQ | 3 |
| Total | 4000+ | |
Our Expertise
We leverage an extensive collection of 200+ purified chemical standards encompassing diverse lipid classes, laying the groundwork for precise lipid identification and retention time pattern evaluation. Augmented by comprehensive references and varied lipid databases, our approach facilitates the accurate discernment of a broad spectrum of lipid compounds.
Employing the gold standard for metabolite quantification—mass spectrometry's multiple reaction monitoring (MRM) detection mode—we guarantee precise lipid quantification. This mode not only enhances accuracy but also enables the detection of minute quantities of lipid-like substances. For instance, in a mouse liver sample, we can detect over 1700 lipids.
The utilization of the MRM mode within mass spectrometry stands as a pivotal element in ensuring the consistent and stable detection of lipids, especially when analyzing extensive sample cohorts.
Sample Requirements of Quantitative Lipidomics Service
|
Sample type |
sample |
Recommended sample |
Minimum sample |
Biological replication |
|
liquid |
Plasma, serum, hemolymph, milk, egg white |
100 μL |
20μL |
human＞30 animal＞8
|
|
Cerebrospinal fluid, tear fluid, interstitial fluid, uterine fluid, pancreatic fluid and bile, pleural effusion, follicular fluid, culture medium |
100 μL |
20μL |
||
|
Seminal plasma, amniotic fluid, prostate fluid, rumen fluid, respiratory condensate, gastric lavage fluid, alveolar lavage fluid, urine, sweat, corpse fluid, saliva, sputum |
500 μL |
100 μL |
||
|
tissue |
Animal tissue, placenta, thrombus, fish skin, mycelium, nematode |
100 mg |
20 mg |
|
|
Whole body, aircraft (wings), pupae |
500 mg |
20 mg |
||
|
Zebrafish organs, insect organs |
20 |
10 |
||
|
cell |
Cultured cells |
2*10^6 |
1*10^6 |
|
|
Escherichia coli and other microorganisms |
1*10^10 |
5*10^8 |
||
|
feces |
Feces,iIntestinal contents |
200 mg (Wet weight) |
50 mg (Wet weight) |
|
