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Metabolomics Service Platform

Triple Quadruple Pole Mass Spectrometer

Triple Quadruple Pole Mass Spectrometer

QTRAP® 6500+ system(20sets)

Resolution Ratio

≥9000

Mass Range

5-2000m/z

Scanning Speed

20000Da/s

Mass Accuracy

<0.01%(Total mass number range)

Sensitivity

ESI Positive ions:1pg Reserpine was injected,S/N≥160000:1

ESI Negative ions:1pg Estradiol injection,S/N≥500:1

Dynamic Range

>10 6

Triple Quadruple Pole Mass Spectrometer

QTRAP® 4500 system(4 sets)

Resolution Ratio

Unit resolution

Mass Range

5-2000m/z

Scanning Speed

12000Da/s

Mass Accuracy

<0.1 Da over 24 hours

Sensitivity

1pg reserpine,S/N≥200,000:1

Dynamic Range

>10 5

High Resolution Mass Spectrometers

High Resolution Mass Spectrometers

Agilent 6545 Q-TOF LC-MS

Mass Resolving Power

45000(FWHM )at m/z 2,722

Mass Range

100–10,000 m/z

Spectral Acquisition Rate

50 spectra/second

Mass Accuracy

Internal standard <0.08ppm

Sensitivity

ESI Positive ions: 1pg Reserpine was injected, S/N≥500:1

Dynamic Range

>10 5

High Resolution Mass Spectrometers

Q ExactiveHF-X

Resolution ratio

240000@m/z 200

Mass Range

50-6000m/z

Scanning Speed

Up to 40Hz

Quality Accuracy

Internal standard<1ppm RMS,

External standard<3ppm RMS

Sensitivity

Full scanning MS: The S/N of reserpine on 100FG upper column was 150:1

SIM:50FG S/N of reserpine on the upper column was 150:1

Dynamic range

>5000:1

High Resolution Mass Spectrometers

Bruker TimsTof Pro

Resolution Ratio

At a high acquisition rate≥(40Hz),

resoluton≥50,000(@M/Z1222,FWHM)

Mass Range

20-40,000 m/z

Scanning Speed

MS≥40 Hz, MS/MS acquisition rate≥100 Hz

Quality Accuracy

Internal standard <0.8ppm RMS,

External standard<2ppm RMS

Sensitivity

Mobility mode: MS:1pg/uL reserpine, S/N>100: 1;

MS/MS:2.5Fmol Glu-fibrinopeptide B, the strongest peak signal in y ion of divalent ion fragments was greater than 800, S/N>100:1

High Resolution Mass Spectrometers

Bruker TimsTof FleX

Resolution Ratio

60,000(@M/Z1222, FSR), independent of scanning speed

Mass Range

20-40,000 m/z

Scanning Speed

MS:50 Hz,

MS/MS:50Hz(Contour plots, peak detection and data storage)

PASEF(ESI):120 Hz

Quality Accuracy

Internal standard : <0.8ppm,

External standard: 2ppm

Sensitivity

MS:1pg/uL reserpine,S/N>100:1;

MS/MS: 2.5fmol Flu-Fibrinopeptide, S/N 50:1;TIMS-MS: 50 fg/ul reserpine, < 15% RSD;

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