Anthocyanin Assay

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Anthocyanidin is a class of water-soluble pigment compounds that widely exist in plants. Free anthocyanins are rare under natural conditions and mainly exist in the form of glycosides. Metware's Anthocyanidin Analysis assay is a targeted LC-MS/MS method that will detect and obtain absolute and semi-quantification of 108 compounds in the anthocyanin metabolic pathway.

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Technology Introduction Applications List of Metabolites Project Workflow

Technology Introduction

Anthocyanin assay is an anthocyanin targeted metabolomics approach with accurate identification and quantification of 108 anthocyanidins, which include pelargonidins, cyanidins, delphinidins, peonidins, petunidins, malvidins, procyanidins, among others.

Technology Introduction

Technical Features of Anthocyanin Assay Service

Wide Coverage

108 anthocyanidin metabolites over 8 different types.

Absolute Quantification

Absolute anthocyanin quantification of 41 metabolites with standard curver > 0.99 and semi-quantification of 67 metabolites.

Applications of Anthocyanin Assay Service

Applications: Research In Plant Color

Applications: Research In Stress Response And Environmental Adaptation

Abiotic stress: light (UV), water (drought, waterlogging), heavy metals (cadmium, lead, etc.), temperature (high temperature,low temperature), mechanical damage and other external factors bring reversible or irreversible effects on plants.

Biological stress: the influence of insects, bacteria, fungi, and viruses on the growth and development of plants.

Article Spotlight: Transcriptomics integrated with metabolomics reveals the effect of regulated deficit irrigation on anthocyanin biosynthesis in Cabernet Sauvignon grape berries.

Regulated deficit irrigation (RDI) is a new type of water-saving irrigation technology developed in recent years which was well suited to arid and semi-arid grape plant areas. The anthocyanin synthesis of grapes under RDI was revealed through omics in this study. RDI slightly decreased the hundred-grain weight and increased the soluble solid content, juice pH, reducing sugar content, and total anthocyanin content. Meanwhile, the total acid content decreased before ripening. Transcriptomics and metabolomics analyses revealed that large numbers of differentially expressed genes (DEGs) and significantly changed metabolites (SCMs) were filtered in the RDI groups. RDI1 with 30% ETc upregulated 7 related gene expression levels in the anthocyanin biosynthetic pathway and also increased some metabolites contents. Eventually, the contents of most monomeric antho cyanins in the RDI groups were increased, and the proportion of Mv increased in the ripe grapes of the RDI groups. In all, RDI is a useful water-saving irrigation method that could also increase anthocyanin content in grapes.

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SERVICES

Global Metabolite Profiling

Untargeted Metabolomics

TM Widely-Targeted Metabolomics

Widely-Targeted Metabolomics for Plants

Flavonoids Metabolomics

Targeted Metabolomics

Bile Acid

Energy Metabolism

Tryptophan Targeted Metabolomics

Amino Acid Targeted Metabolomics

Short-Chain Fatty Acids

Plant Hormone Assay

Carotenoid Targeted Metabolomics

Anthocyanin Assay

Gibberellin Assay

Lipidomics

Quantitative Lipidomics

Quantitative Lipidomics for Plants

Proteomics

DIA Quantitative Proteomics

DDA Quantitative Proteomics

Serum/Plasma Quantitative Proteomics

Phosphoproteomics

Multi-Omics

Proteomics + Metabolomics

Microbiome+Metabolome

Transcriptome+Metabolome

Resequencing+Metabolome

Transcriptomics + Proteomics + Metabolomics

Eukaryotic mRNA-Seq

16S rRNA gene Sequencing

Metagenomic Sequencing

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List of Metabolites

Anthocyanin	database	v2.0
NO.	Compounds	Class	Quantitative
1	Cyanidin-3-(6''-caffeylsophoroside)-5-glucoside	Cyanidin	Semiquantitative
2	Cyanidin-3-(6-O-p-caffeoyl)-glucoside	Cyanidin	Semiquantitative
3	Cyanidin-3,5,3'-O-triglucoside	Cyanidin	Semiquantitative
4	Cyanidin-3,5-O-diglucoside	Cyanidin	Semiquantitative
5	Cyanidin-3-O-(6''-ferulylsophoroside)-5-glucoside	Cyanidin	Semiquantitative
6	Cyanidin-3-O-(6-O-malonyl-beta-D-glucoside)	Cyanidin	Semiquantitative
7	Cyanidin-3-O-5-O-(6-O-coumaroyl)-diglucoside	Cyanidin	Semiquantitative
8	Cyanidin-3-O-galactoside	Cyanidin	Semiquantitative
9	Cyanidin-3-O-glucoside	Cyanidin	Semiquantitative
10	Cyanidin-3-O-rutinoside	Cyanidin	Semiquantitative
11	Cyanidin-3-O-rutinoside-5-O-glucoside	Cyanidin	Semiquantitative
12	Cyanidin-3-O-sambubioside-5-O-glucoside	Cyanidin	Semiquantitative
13	Cyanidin-3-O-sophoroside	Cyanidin	Semiquantitative
14	Cyanidin-3-O-(6-O-p-coumaroyl)-glucoside	Cyanidin	Absolute quantification
15	Cyanidin-3-O-arabinoside	Cyanidin	Absolute quantification
16	Cyanidin-3-O-sambubioside	Cyanidin	Absolute quantification
17	Cyanidin-3-O-xyloside	Cyanidin	Absolute quantification
18	Delphinidin-3-O-(6-O-acetyl)-glucoside	Delphinidin	Semiquantitative
19	Delphinidin-3-O-(6-O-malonyl)-glucoside-3'-glucoside	Delphinidin	Semiquantitative
20	Delphinidin-3-O-rhamnoside	Delphinidin	Semiquantitative
21	Delphinidin-3-O-rutinoside	Delphinidin	Semiquantitative
22	Delphinidin-3-O-rutinoside-5-O-glucoside	Delphinidin	Semiquantitative
23	Delphinidin-3-O-sambubioside	Delphinidin	Semiquantitative
24	Delphinidin-3-O-sambubioside-5-O-glucoside	Delphinidin	Semiquantitative
25	Delphinidin-3-O-sophoroside	Delphinidin	Semiquantitative
26	Delphinidin-3,5-O-diglucoside	Delphinidin	Absolute quantification
27	Delphinidin-3-O-(6-O-malonyl-beta-D-glucoside)	Delphinidin	Absolute quantification
28	Delphinidin-3-O-(6-O-p-coumaroyl)-glucoside	Delphinidin	Absolute quantification
29	Delphinidin-3-O-5-O-(6-O-coumaroyl)-diglucoside	Delphinidin	Absolute quantification
30	Delphinidin-3-O-arabinoside	Delphinidin	Absolute quantification
31	Delphinidin-3-O-galactoside	Delphinidin	Absolute quantification
32	Delphinidin-3-O-glucoside	Delphinidin	Absolute quantification
33	Delphinidin	Delphinidins	Absolute quantification
34	Kaempferol-3-O-rutinoside	flavonoid	Semiquantitative
35	Quercetin-3-O-glucoside	flavonoid	Semiquantitative
36	Afzelin	flavonoid	Absolute quantification
37	Chalcone	flavonoid	Absolute quantification
38	Dihydrokaempferol	flavonoid	Absolute quantification
39	Dihydromyricetin	flavonoid	Absolute quantification
40	Naringenin	flavonoid	Absolute quantification
41	Naringenin-7-O-glucoside	flavonoid	Absolute quantification
42	Rutin	flavonoid	Absolute quantification
43	Malvidin-3-O-(6-O-p-coumaroyl)-glucoside	Malvidin	Semiquantitative
44	Malvidin-3-O-5-O-(6-O-coumaroyl)-diglucoside	Malvidin	Semiquantitative
45	Malvidin-3-O-arabinoside	Malvidin	Semiquantitative
46	Malvidin-3-O-galactoside	Malvidin	Semiquantitative
47	Malvidin-3-O-sambubioside-5-O-glucoside	Malvidin	Semiquantitative
48	Malvidin-3-O-sophoroside	Malvidin	Semiquantitative
49	Malvidin	Malvidin	Absolute quantification
50	Malvidin-3,5-O-diglucoside	Malvidin	Absolute quantification
51	Malvidin-3-O-(6''-acetylglucoside)-5-glucoside	Malvidin	Absolute quantification
52	Malvidin-3-O-(6-O-malonyl-beta-D-glucoside)	Malvidin	Absolute quantification
53	Malvidin-3-O-glucoside	Malvidin	Absolute quantification
54	Malvidin-3-O-rutinoside	Malvidin	Absolute quantification
55	Malvidin-3-O-sambubioside	Malvidin	Absolute quantification
56	Pelargonidin	Pelargonidin	Semiquantitative
57	Pelargonidin-3-(6''-caffeylsophoroside)-5-glucoside	Pelargonidin	Semiquantitative
58	Pelargonidin-3,5-O-diglucoside	Pelargonidin	Semiquantitative
59	Pelargonidin-3-O-(6''-ferulylsambubioside)-5-O-(malonyl)-glucoside	Pelargonidin	Semiquantitative
60	Pelargonidin-3-O-(6-O-malonyl-beta-D-glucoside)	Pelargonidin	Semiquantitative
61	Pelargonidin-3-O-(6-O-p-coumaroyl)-glucoside	Pelargonidin	Semiquantitative
62	Pelargonidin-3-O-[2-O-glucosyl-6-O-p-coumaroyl-glucoside]-5-O-glucoside	Pelargonidin	Semiquantitative
63	Pelargonidin-3-O-[6-O-feruloyl-2-O-glucosyl-glucoside]-5-O-glucoside	Pelargonidin	Semiquantitative
64	Pelargonidin-3-O-5-O-(6-O-coumaroyl)-diglucoside	Pelargonidin	Semiquantitative
65	Pelargonidin-3-O-arabinoside	Pelargonidin	Semiquantitative
66	Pelargonidin-3-O-galactoside	Pelargonidin	Semiquantitative
67	Pelargonidin-3-O-glucoside	Pelargonidin	Semiquantitative
68	Pelargonidin-3-O-rutinoside-5-O-glucoside	Pelargonidin	Semiquantitative
69	Pelargonidin-3-O-sambubioside	Pelargonidin	Semiquantitative
70	Pelargonidin-3-O-sambubioside-5-O-glucoside	Pelargonidin	Semiquantitative
71	Pelargonidin-3-O-sophoroside	Pelargonidin	Semiquantitative
72	Pelargonidin-3-O-rutinoside	Pelargonidin	Absolute quantification
73	Pelargonidin-3-O-sophoroside-5-O-(malonyl)-glucoside	Pelargonidin	Absolute quantification
74	Pelargonidin-3-sophoroside-5-glucoside	Pelargonidin	Absolute quantification
75	Peonidin	Peonidin	Semiquantitative
76	Peonidin-3-(caffeoyl-glucosyl-glucoside)-5-glucoside	Peonidin	Semiquantitative
77	Peonidin-3-O-(6-O-p-coumaroyl)-glucoside	Peonidin	Semiquantitative
78	Peonidin-3-O-arabinoside	Peonidin	Semiquantitative
79	Peonidin-3-O-caffeoyl-feruloyl-sophoroside-5-glucoside	Peonidin	Semiquantitative
80	Peonidin-3-O-galactoside	Peonidin	Semiquantitative
81	Peonidin-3-O-glucoside	Peonidin	Semiquantitative
82	Peonidin-3-O-P-hydroxybenzoylsophoroside-5-glucoside	Peonidin	Semiquantitative
83	Peonidin-3-O-rutinoside	Peonidin	Semiquantitative
84	Peonidin-3-O-sambubioside	Peonidin	Semiquantitative
85	Peonidin-3-O-sambubioside-5-O-glucoside	Peonidin	Semiquantitative
86	Peonidin-3-sophoroside-5-glucoside	Peonidin	Semiquantitative
87	Peonidin-3,5-O-diglucoside	Peonidin	Absolute quantification
88	Peonidin-3-O-(6''-ferulylsophoroside)-5-glucoside	Peonidin	Absolute quantification
89	Peonidin-3-O-(6-O-malonyl-beta-D-glucoside)	Peonidin	Absolute quantification
90	Peonidin-3-O-5-O-(6-O-coumaroyl)-diglucoside	Peonidin	Absolute quantification
91	Peonidin-3-O-sophoroside	Peonidin	Absolute quantification
92	Petunidin-3-O-(6-O-p-coumaroyl)-glucoside	Petunidin	Semiquantitative
93	Petunidin-3-O-5-O-(6-O-coumaroyl)-diglucoside	Petunidin	Semiquantitative
94	Petunidin-3-O-arabinoside	Petunidin	Semiquantitative
95	Petunidin-3-O-glucoside	Petunidin	Semiquantitative
96	Petunidin-3-O-sambubioside	Petunidin	Semiquantitative
97	Petunidin-3,5-O-diglucoside	Petunidin	Absolute quantification
98	Petunidin-3-O-(6-O-malonyl-beta-D-glucoside)	Petunidin	Absolute quantification
99	Petunidin-3-O-galactoside	Petunidin	Absolute quantification
100	Petunidin-3-O-rutinoside	Petunidin	Absolute quantification
101	Petunidin-3-O-sambubioside-5-O-glucoside	Petunidin	Absolute quantification
102	Petunidin-3-O-sophoroside	Petunidin	Absolute quantification
103	Procyanidin B3	Procyanidin	Semiquantitative
104	Procyanidin A1	Procyanidin	Absolute quantification
105	Procyanidin A2	Procyanidin	Absolute quantification
106	Procyanidin B1	Procyanidin	Absolute quantification
107	Procyanidin B2	Procyanidin	Absolute quantification
108	Procyanidin C1	Procyanidin	Absolute quantification

Project Workflow of Anthocyanin Assay Service

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