Steroid Hormone Targeted Metabolomics
Steroid Hormone Targeted Metabolomics
Comprehensive Coverage: Targeting 43 compounds across key classes
Absolute Quantification: 43 standard curves with r>0.99
High Sensitivity: Detecting at ng/ml concentrations
Rigorous Quality Control: Ensuring data reliability
Technology Introduction of Steroid Hormones Analysis
Steroid hormones are a group of hormones derived from cholesterol that play vital roles in regulating various physiological processes, including androgens (e.g., testosterone), estrogens (e.g., estradiol), progestogens (e.g., progesterone), glucocorticoids (e.g., cortisol), and mineralocorticoids (e.g., aldosterone). Primarily produced by the gonads (ovaries and testes) and adrenal glands, these lipid-soluble hormones can easily cross cell membranes and bind to specific receptors within target cells. They are essential in processes such as sexual differentiation, reproduction, stress response, immune function, and the regulation of fluid and electrolyte balance.
MetwareBio’s Steroid Hormones Targeted Metabolomics Service delivers precise absolute quantification of 43 steroid hormones, employing a dual-validation approach with both internal and external standards, powered by our comprehensive proprietary database.
Steroid Hormones Targeted Metabolomics Service technology workflow
Technology Superiority of Steroid Hormones Analysis
Comprehensive Coverage
Targeting 43 compounds across key classes, including androgens, estrogens and progestogens.
Absolute Quantitation
43 standard curves with r>0.99.
High Sensitivity
Utilizes the AB QTRAP® 6500+ LC-MS/MS, enabling detection at ng/ml concentrations.
Rigorous Quality Control
Ensuring data reliability with controls like blanks, solvents, and mixed standards.
Applications of Steroid Hormones Targeted Metabolomics
Disease Mechanism
Steroid Hormones Targeted Metabolomics Analysis can aid in understanding the underlying mechanisms of diseases by providing precise quantification of steroid hormones involved in conditions like hormonal imbalances, metabolic disorders, and hormone-sensitive cancers, helping to uncover disease pathways and potential therapeutic targets.
Drug Development
Steroid Hormones Targeted Metabolomics Analysis can support drug development by enabling the identification and quantification of steroid hormones, which are crucial for assessing the impact of potential treatments on hormonal regulation, efficacy, and safety. It also aids in evaluating the effects of new compounds on steroid hormone-related pathways in preclinical and clinical trials.
Biomarker Discovery
Steroid Hormones Targeted Metabolomics Analysis can enhance biomarker discovery by providing exceptional sensitivity and accuracy in measuring steroid hormones. It enables the identification of potential biomarkers for early diagnosis, prognosis, and monitoring of diseases, particularly in areas like cancer, metabolic disorders, and reproductive health.
Personalized Medicine
Steroid Hormones Targeted Metabolomics Analysis can play a key role in personalized medicine by providing detailed hormone profiling to tailor treatment strategies based on individual hormonal patterns. This is particularly useful in optimizing therapies for conditions such as infertility, hormone-sensitive cancers, and metabolic disorders, ensuring more effective and individualized patient care.
SERVICES
Bile Acid
Oxylipin Targeted Metabolomics
Neurotransmitter Targeted Metabolomics
Steroid Hormone Targeted Metabolomics
Energy Metabolism
Tryptophan Targeted Metabolomics
Amino Acid Targeted Metabolomics
Short-Chain Fatty Acids
Plant Hormone Assay
Carotenoid Targeted Metabolomics
Anthocyanin Assay
Gibberellin Assay
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List of steroid Molecules
| Index | Compound | CAS No. |
|---|---|---|
| 1 | Testosterone | 58-22-0 |
| 2 | Dihydrotestosterone | 521-18-6 |
| 3 | Androsterone | 53-41-8 |
| 4 | 5β-Androsterone | 53-42-9 |
| 5 | Cortisol | 50-23-7 |
| 6 | Deoxycorticosterone | 64-85-7 |
| 7 | Corticosterone | 50-22-6 |
| 8 | Aldosterone | 52-39-1 |
| 9 | Cortisone | 53-06-5 |
| 10 | 17a-Estradiol | 57-91-0 |
| 11 | Estrone | 53-16-7 |
| 12 | 17α-Ethinylestradiol | 57-63-6 |
| 13 | 2-Methoxy Estrone | 362-08-3 |
| 14 | 2-Hydroxy-Estrone | 362-06-1 |
| 15 | Pregnenolone | 145-13-1 |
| 16 | 17α-Hydroxypregnenolone | 387-79-1 |
| 17 | Progesterone | 57-83-0 |
| 18 | 17a-Hydroxyprogesterone | 68-96-2 |
| 19 | Pregnanediol | 80-92-2 |
| 20 | Cholesterol | 57-88-5 |
| 21 | Desmosterol | 313-04-2 |
| 22 | Lathosterol | 80-99-9 |
| 23 | 7-Ketocholesterol | 566-28-9 |
| 24 | β-Sitosterol | 83-46-5 |
| 25 | Vitamin D3 | 67-97-0 |
| 26 | … | … |
Contact for a full list.
Sample Requirement of Steroid Hormones Targeted Metabolomics
| Sample Class | Sample Type | Recommended Sample Size |
Minimum Sample Size |
| Liquid I | Plasma, Serum, Hemolymph, Whole Blood, Milk, Egg White | 100 μl | 50 μl |
| Liquid II | Cerebrospinal Fluid (CSF), Interstitial Fluid (TIF), Uterine Fluid, Pancreatic Juice, Bile, Pleural Effusion, Follicular Fluid, Postmortem Fluid, Tissue Fluid, Culture Medium (liquid), Culture Supernatant, Tears, Aqueous Humor, Digestive Juices, Bone Marrow (liquid) | 100 μl | 50 μl |
| Liquid Ⅲ | Seminal Plasma, Amniotic Fluid, Prostatic Fluid, Rumen Fluid, Respiratory Condensate, Gastric Lavage Fluid, Bronchoalveolar Lavage Fluid (BALF), Urine, Sweat, Saliva, Sputum | 500 μl | 100 μl |
| Tissue I | Small Animal Tissues, Placenta, Blood Clot, Mycelium, Nematode, Zebrafish (whole fish), Bone Marrow (solid), Nail | 100 mg | 20 mg |
| Tissue II | Large Animal Tissues, Whole Insect Body, Wings (of insects), Pupa, Eggs, Large Fungi (mushroom types), Large Algae (red algae), Large Amount of Mycelium/Mycelial Balls, Cartilage, Bone (solid) | 500 mg | 20 mg |
| Tissue Ⅲ | Zebrafish Organs, Insect Organs, Whole Microinsect Body (e.g., Drosophila) | 20 units | 10 units |
| Solid I | Feces, Intestinal Contents, Lyophilized Fecal Powder | 200 mg | 20 mg |
| Solid II | Milk Powder, Microbial Fermentation Product (solid), Culture Medium (solid), Earwax, Lyophilized Tissue Powder, Feed, Egg Yolk, Lyophilized Plant Powder, Lyophilized Egg Powder | 100 mg | 20 mg |
| Solid Ⅲ | Honey, Nasal Mucus, Sputum | 100 mg | 20 mg |
| Solid Ⅳ | Sludge, Soil | 600 mg | 300 mg |
| Cell I | Adherent Cells, Animal Cell Lines | 1×107 cells | 1×106 cells |
| Cell II | E. Coli, Yeast Cells | 1×1010 cells | 5×108 cells |
| Cell Ⅲ | Small Amount of Fungal Mycelial Balls/Mycelium, Unicellular Algae (Cyanobacteria), Large Quantities of Bacterial Hyphae (sediment), Mucilaginous Protoplasmic Clusters (hyphae) | 100 mg | 20 mg |
| Organelle I | Lysosomes, Mitochondria, Endoplasmic Reticulum | 4×107 cells | 1×107 cells |
| Organelle II | Exosomes, Extracellular Vesicles | 2×109 particles | 1×109 particles |
| Special Sample I | Skin Tape or Patch | 2 pieces | 1 piece |
| Special Sample II | Test Strips | 2 pieces | 1 piece |
| Special Sample Ⅲ | Swab | 1 piece | 1 piece |
Case Study of Steroid Hormones Targeted Metabolomics
Article: Hypothalamic pregnenolone mediates recognition memory in the context of metabolic disorders
Summary
Obesity and type 2 diabetes are associated with cognitive dysfunction. Because the hypothalamus is implicated in energy balance control and memory disorders, we hypothesized that specific neurons in this brain region are at the interface of metabolism and cognition. Acute obesogenic diet administration in mice impaired recognition memory due to defective production of the neurosteroid precursor pregnenolone in the hypothalamus. Genetic interference with pregnenolone synthesis by Star deletion in hypothalamic POMC, but not AgRP neurons, deteriorated recognition memory independently of metabolic disturbances. Our data suggest that pregnenolone’s effects on cognitive function were mediated via an autocrine mechanism on POMC neurons, influencing hippocampal long-term potentiation. The relevance of central pregnenolone on cognition was also confirmed in metabolically unhealthy patients with obesity. Our data reveal an unsuspected role for POMC neuron-derived neurosteroids in cognition. These results provide the basis for a framework to investigate new facets of POMC neuron biology with implications for cognitive disorders.
Reference
Ramírez S, Haddad-Tóvolli R, Radosevic M, et al. Hypothalamic pregnenolone mediates recognition memory in the context of metabolic disorders. Cell Metab. 2022;34(2):269-284.e9. https://www.cell.com/cell-metabolism/fulltext/S1550-4131(21)00689-6
SERVICES
Bile Acid
Oxylipin Targeted Metabolomics
Neurotransmitter Targeted Metabolomics
Steroid Hormone Targeted Metabolomics
Energy Metabolism
Tryptophan Targeted Metabolomics
Amino Acid Targeted Metabolomics
Short-Chain Fatty Acids
Plant Hormone Assay
Carotenoid Targeted Metabolomics
Anthocyanin Assay
Gibberellin Assay
WHAT'S NEXT IN OMICS: THE METABOLOME
Please submit a detailed description of your project. We will provide you with a customized project plan metabolomics services to meet your research requests. You can also send emails directly to support-global@metwarebio.com for inquiries.
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