Lipids for Lipidomics FAQ

Can you detect linear fatty acids?

Yes, our short-chain fatty acid assay can detect some short-chain fatty acids, including acetic acid, propionic acid, and butyric acid. Additionally, our quantitative lipidomics panel is capable of detecting a wide range of long-chain linear fatty acids.

What types of lipids can you detect in yoru lipidomics panel?

In our lipidomics panel, we can detect a wide variety of lipid types based on our lipid database containing 3000+ lipids, including fatty acyls (FA) such as acylcarnitines and free fatty acids, glycerolipids (GL) like monoacylglycerols (MG), diacylglycerols (DG), and triacylglycerols (TG), glycerophospholipids (GP) such as phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylserine (PS), sphingolipids (SP) like ceramides and sphingosine, sterol lipids (ST) including sterols and bile acids (BA), and prenol lipids (PR) such as coenzyme Q (CoQ).

what CASE stands for in our quantitative lipidomics database table

It stands for Sitosterol ester

Have we worked with before or can we work with poultry egg samples?

yes, we have experiences on yolk with untargeted metabolomics and wildy targeted metabolomics analysis, and over-all metabolomics, quantative lipidomics.

Can we identify any DGMG and MGMG in our Lipidomics panel?

yes, we can identify some MGDG and DGDG for Lipidomics analysis.

Can we detect tryglycerides in Quantitative Lipidomics? We are concerned that tryglycerides could breakdown due to its structure.

We can detect tryglycerides in Quantitative Lipidomics.Tryglycerides are stable and not easy to breakdown.

Can we also detect steroids like cholesterol in TM? I know quantitative lipidomics database has it but the client also wants to know if TM can detect that as well

No, we can not detect steroids like cholesterol in TM. Sterols are challenging to detect using the Widely-Targeted Metabolomics for Plants method. Even when detected in limited quantities, they tend to exhibit instability and poor peak performance

We are interested in Lactosylceramide and Ceramide-1-Phosphate, please also check if these compounds are detectable using our quantitative lipidomics process, and if they have been detected in our past projects.

We can detect Lactosylceramide and Ceramide-1-Phosphate using our quantitative lipidomics process.

We are interested in comparing the composition of LPS (or Lipid A specifically) between wild type and mutant Salmonella strains. Are we able to detect LPS (Lipopolysaccharides)?

The MS used in our lipid analysis is suitable for the detection of small molecules with molecular weights below 1200 Da, rendering us unable to detect lipopolysaccharides and lipid A. Nonetheless, our expertise is well-established in the detection of other small-molecule metabolites. Should you have additional requirements, we welcome the opportunity for collaboration and encourage you to reach out to us.

I have been looking for help running approximately 1000 samples for targeted lipidomics. I am interested in FFA, sphyngolipids, phospholipids, and eicosanoids. I suspect it would likely be in 2 batches, but if the cost is substantially different, we may be able to do in one batch.

For the metabolites you are interested in, encompassing FFA, Sphingolipids, Glycerophospholipids, and Eicosanoids, we boast an extensive and reliable database, backed by substantial project experience. Within the Fatty Acyls category, inclusive of FFA and Eicosanoids, our database contains 270 distinct lipids. Furthermore, for Sphingolipids and Glycerophospholipids, we have comprehensive data on 1800 and 828 lipids, respectively. Across all lipid classes, our database spans over 4000 lipids. We have detection results from our quantitative lipid projects using human blood samples, featuring an average detection of 1140 lipids across 52 lipid classes. Specifically, for FFA, Sphingolipids, Glycerophospholipids, and Eicosanoids, we offer information on 30 detectable lipids per class for your reference.

Do you have the information of our experience with mouse cells. We are looking for a targeted aqueous panel and acylcarnitine analysis in mouse bone marrow derived macrophages.

For the substance of interest to the client-acylcarnitine, we have extensive experience in the quantitative lipidomics panel. We can provid the detected types of acylcarnitines and detection rates in four quantitative lipidomics projects with mouse cell samples.

We are interested in determing the lipidomics from lipid droplets isolated from mice livers (treated and controls). And we want to determine if the quantity and type of lipids in LDs change due to the treatment.

We have experience on human lipid droplets samples and mouse liver samples in QL panel.

We has a project which will require the analysis of a large cohort of patient samples. I need some experiences on lipidomics analyses for CSF (Cerebrospinal fluid), Serum, human brain tissue and iPSC-neuron cultures.

We have experience on human CSF (Cerebrospinal fluid), human serum, mouse brain tissue and human induced neuron cells in QL panel.

How many mitochondrial related lipids are in quantitative lipidomics panel? We are interested in mitochondrial lipids.

Mitochondria are capable of synthesizing several lipids autonomously such as phosphatidylglycerol, cardiolipin and in part phosphatidylethanolamine, phosphatidic acid and CDP-diacylglycerol. For the phosphatidylglycerol, phosphatidylethanolamine, phosphatidic acid, we are able to detect these lipids. Our QL panel encompasses over 4,000 lipids and we are able to detect detect 800-1,300 lipids in biological samples.

Can you also provide some of our experiences working with human serum and plasma on the lipids in this list using Quantitative Lipidomics panel?

There are 19 lipids in the given list. Among these, 14 lipids are included in our QL database, with 10 of them detected in QL projects using human plasma/serum samples. It's important to note that for monoglycerides (MGs) in our QL database, we can only ascertain the carbon chain length and the number of double bonds. The specific location of the fatty acid chain remains unknown. Therefore, when searching in the QL database, we use MG(20:4) to represent MG(0:0/20:4/0:0), MG(20:4/0:0/0:0), or MG(0:0/0:0/20:4). This convention applies to other MGs as well.

1) Does the quantitative lipidomics targets simple lipids? 2) Which method would be better to study short chain fatty acids? Targeted metabolomics or quantitative lipidomics? 3) Are indole covered in the untargeted metabolomics? If not, is there a targted metabolomics with this of focus?

The QL can detect many lipids. SCFA is best detected using our SCFA targeted assay. It is not captured using QL or untargeted approach. Tryptophan and Indole are part of our database. That being said, Indole is known to be challenging to detect and the detection rate is low.

Can our Lipidomics capture the following fatty acids: Linoleic acid and 9-Hydroxyoctadecadienoic acid. If not by Quantitative Lipidomics, can Untargeted or TM capture these compounds? Sample type: Human cells, plasma/serum. What is Linoleic acid called in our QL panel?

Linoleic acid can be detected in quantitative lipidomics, TM Widely-Targeted Metabolomics and untarged metabolomics Panels with human cell or blood samples. 9-Hydroxyoctadecadienoic acid cannot be detecte in quantitative lipidomics, but can be detected in TM and untarged panels with human cell or blood samples. Linoleic acid called FFA(18:2) in our quantitative lipidomics panel; 9-Hydroxyoctadecadienoic acid called 9(S)-HODE in our TM panel and alpha-Dimorphecolic acid in our Untargeted Panel.

Are you able to detect plasmalogen ether phospholipids? For example: C18(Plasm)-18:1 PE, 1-O-1'-(Z)-octadecenyl-2--(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine. What is C18(Plasm)-18:1 PE called in our QL panel?

We are able to detect C18(Plasm)-18:1 PE in our QL panel. This compound belongs to PE-P (secondary class) of GP (primary class) in our QL database and we can detect many PE-Ps in the QL panel.

We are interested in sphingolipids for plants. Can you provide a list of sphingolipids from your product?

Yes, we can provide a demo list of sphingolipids detected in plant lipidomics panel.

How to measure 4-hydroxynonenal (4-HNE) or other byproducts of lipid peroxidation?

4-hydroxynonenal (4-HNE) can be detected in our widely-targeted metabolomics panel.

We want to detect compounds: Arachidonic acid (AA) and Prostaglandin E2 (PGE2);

AA and PGE2 can be detected in our quantitative lipidomics, TM widely-targeted metabolomics and untargeted metabolomics panels.

We are interested in lipidomics panel, however, we found that your company used HexCer, Sph, Cert, and Cer to categorize plant sphingolipids, but plant sphingolipids are not categorized in this way. Plants contain ceramide, glycosyl ceramide, inositol phosphoryl ceramide, and glycosyl inositol phosphoryl ceramide

we have ceramide (Cer) and glycosyl ceramide (HexCer) in our plant QL database. But inositol phosphoryl ceramide (IPC) and glycosyl inositol phosphoryl ceramide (GIPC) are absent.

We are interested in targeting the citric acid cycle and lipids, but we only want to proceed in one type of service. Is it possible to only use Widely Targeted service to analysis lipids and TCA metabolites?

I would recommend Untargeted Plus. We can capture the TCA cycle metabolites better, but not guaranteed. We can capture some lipids, but we will still recommend lipidomics for the best lipid analysis.

Would you please check whether these compouds are in our lipidomics panel? Linoleic Acid, Lipoic Acid, Arachidonic Acid, Cholesterol, DL-alpha Tocopherol-acetate, Linolenic Acid, Myristic Acid, Oleic Acid, Palmitic Acid, Palmitoleic Acid, Stearic acid and cis-4,7,10,13,16,19-Docosahexaenoic acid.

Yes, they are all included in our quantitative lipidomics panel, except Lipoic Acid and DL-alpha Tocopherol-acetate.

Does your Quantitative Lipidomics panel contain free fatty acids 16:0 and 18:1?

yes. they are common lipids which have been included in our lipidomics panel

Can you detect long chain dicarboxylic acids (C16-C20)? If so, can you provide some compounds in our database?

Hexadecanedioic acid (C16) and Octadecanedioic acid (C18) can be detected in our widely-targeted metabolomics and untargeted metabolomics panels.

I am interested in detecting a couple of lipid classes (triglycerides and cholesteryl esters) along with other metabolites such as carbohydrate. What will be the best method to capture these?

Our quantitative lipidomics panel is able to cover the lipids as concerned.

Are we able to detect 3-ketosphinganine and sphinganine using quantitative lipidomics? If not, are they in untargeted targeted panel?

Sphinganine can be detected using widely-targeted metabolomics, untargeted metabolomics and quantitative lipidomics panels. 3-ketosphinganine can be detected using widely-targeted metabolomics and untargeted metabolomics assays.

How to obtain the ratio of Omega 3 fatty acid to Omega 6 fatty acid in fish fillet tissues. What are the specific Omega 3 fatty acid and Omega 6 fatty acid compounds in our quantitative lipidomics?

Since we cannot precisely identify the positions of double bonds on the carbon chain, we are unable to determine the exact number of ω3 and ω6 fatty acids in our QL database, which are classified based on the location of the first double bond from the methyl end. However, we verified the presence of well-known ω3 and ω6 fatty acids, including FFA (18:3) containing ALA, FFA (20:5) containing EPA, FFA (22:6) containing DHA, FFA (18:2) containing LA, and FFA (20:4) containing AA.

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