Global metabolite profiling via LC-MS is often used for detecting metabolites from biological or environmental samples and obtaining relative quantification in an unbiased manner. The main application of lcms metabolomics is to compare the case group with the control group to find metabolites that show significant differences and thereby providing clues to the metabolic pathways and genes that are involved in the process. Metabolomics pathway analysis is often the first metabolomics assessment in biomarker development, disease pathogenesis, and drug development. Aside from the commonly known Untargeted Metabolomics, Metware offers a unique Widely-Targeted Metabolomics workflow that combines high resolution, wide coverage, high sensitivity and precise quantification by applying high-resolution mass spectrometry with QQQ MRM scanning in tandem.