Gibberellin Assay
Gibberellin Assay
Gibberellins (GA) are phytohormones involved chiefly in shoot extension. As a plant growth regulator, GA have been widely used in agricultural production and played an important role in promoting seed germination, stalk elongation, fruit development, and improving plant stress tolerance. Our Gibberellin Assay offers late 2023.
Technology Introduction for Gibberellins
GA can promote plant cell elongation, stem elongation, leaf expansion, accelerated growth and development, early crop maturation, and increase yield or improve quality; it can break dormancy and promote germination; reduce organ abscission, improve fruit set or form seedless fruits, etc. Gibberellins can counteract genetic dwarfism in dwarf pea plants, leading to elongated internodes. Gibberellins act as endogenous growth regulators in plant biosynthesis and were historically discovered as endogenous plant growth regulators. The evolutionary development of gibberellin biosynthesis and signaling pathways in higher plants is significant. Gibberellins are naturally occurring plant hormones produced by specific fungi and plants. The significance of gibberellins as plant growth regulators in agricultural practices is well-documented. Metware Biotechnology has established a method for the analysis of gibberellins based on LC-MS/MS platform with the isotopic internal standard for absolute quantification of 16 GAs for basic life science research.
Technical Features of Gibberellin Assay
Quantitative Accuracy
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11 types of internal standards are used to monitor the whole extraction and detection process.
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The RSD of 80% of substances less than 20% in three technical replicates, and 100% of them RSD are less than 30%.
Ultra-sensitive
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The derivatization process improves the separation and sensitivity of different kinds of erythromycin detection.
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The gibberellin detection line reaches 1.05pg/mL and the lower limit of quantification reaches 3.50pg/mL.
Rigorous Quality Control
18 kinds of erythromycin assays, covering the main substances on the pathway.
High Recognition
The method has been published by customers in more than 100 articles in Nat Commun, Plant Cell, PNAs, Plant Journal, Plant Physiology, Molecular Plant, Scientific Reports, Nature plant, and other journals.
Applications of Gibberellin Assay Service
- Promoting stem elongation;
- Regulating the process of seed germination, the diversity and biological functions of gibberellins across various plant species highlight their role as endogenous growth regulators;
- Promoting the development of floral organs;
- Regulating the development of meristem;
- Regulation of abiotic and biotic stresses;
- Interacting with other hormones Actions.
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Project Workflow of Gibberellin Assay Service
Examples of Gibberellin Functions
| Subdivision | Compound | Genes or cell proteins | Function | Species | References |
| Trichome formation | GibberellinA9 | SlbHLH95/Sl GA20ox2/ SlKS5 |
Positive regulation of the formation of trichome | Tomato | Overexpression of bHLH95, a basic helix-loop-helix transcription factor family member, impacts trichome formation via regulating gibberellin biosynthesis in tomato |
| GibberellinA4 | |||||
| Florescence | Gibberellin | DELLA/FT/ FLC/ SOC1/LFY |
Promotion of plant flowering | Arabidopsis thaliana | New insights into gibberellin signaling in regulating flowering in Arabidopsis |
| Tiller and plant height | Gibberellin | MOC1/SLR1/ TAD1/SLR1 |
Resulting in stem elongation and reduced number of tillers | Rice | SLR1 inhibits MOC1 degradation to coordinate tiller number and plant height in rice |
| Endosperm development | Gibberellin acid | GA20ox/ GA3ox/EUI/ EUIL3 |
Promotion of seed elongation | Rice | Phytohormone dynamics in developing endosperm influence rice grain shape and quality |
| Seed germination | GibberellinA4 | mGA3ox1/Gm KAO/GmGA3 |
Shade environment is regulating GA synthesis | Soybean | Shading in mother plant during seed development promotes subsequent seed germination in soybean |
| Gibberellin acid | REVEILLE1/ RGL2 |
Dormancy control and germination promotion | Arabidopsis thaliana | Interplay between REVEILLE1 and RGA-LIKE2 regulates seed dormancy and germination in Arabidopsis | |
| Gibberellin acid | GASA4、 GASA6 |
Antagonizing the inhibitory effect of melatonin on seed germination | Arabidopsis thaliana | Melatonin inhibits seed germination by crosstalk with abscisic acid, gibberellin, and auxin in Arabidopsis | |
| Blue Light | GibberellinA3 | CRYs/DELLA /SCFSLY1/ GID2 |
Promoting photomorphic construction of plants | Arabidopsis thaliana | Blue light-dependent interactions of CRY1 with GID1and DELLA proteins regulate gibberellin signaling and photomorphogenesis in Arabidopsis |
| Floods | GibberellinA1、GibberellinA4 | ACE1/ACL1/ DCE1 |
Promoting internode elongation | Rice | Antagonistic regulation of the gibberellic acid response during stem growth in rice |
| Glycosylation(leaf regeneration) | GibberellinA3 | PtoWRKY 42 -PtoUGT76C1-1 |
Not significantly correlated with shoot regeneration | Mao Baiyang | Integration of genome wide association studies and co-expression networks reveal roles of PtoWRKY 42-PtoUGT76C1-1 in trans-zeatin metabolism and cytokinin sensitivity in poplar |
WHAT'S NEXT IN OMICS: THE METABOLOME
Please submit a detailed description of your project. We will provide you with a customized project plan metabolomics services to meet your research requests. You can also send emails directly to support-global@metwarebio.com for inquiries.
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