DDA Quantitative Proteomics
DDA Quantitative Proteomics
Accurate qualitative and quantitative analysis with excellent data stability.
High sensitivity with more low-abundance proteins detection.
High throughput, suitable for cohort studies with large sample amounts.
Small sample size, suitable for clinical projects with limited sample availability.
Technology Introduction
Label-free quantitative proteomics technology utilizes the unique dual TIMS design of the timsTOF series mass
spectrometer and the ddaPASEF® acquisition mode to achieve protein qualitative and quantitative analysis. The
ddaPASEF acquisition mode combines data-dependent acquisition (DDA) with PASEF (Parallel Accumulation–Serial
Fragmentation) technology. In the ddaPASEF acquisition mode, precursor ions with high ion intensity are selected
from the MS1 spectra to set the mass spectrum scan range to specific narrow mass windows. Subsequently, the selected
target precursor ions are sequentially fragmented in the MS2 spectra, recording their retention time, mass-to-charge
ratio, ion intensity, and ion mobility. Finally, protein qualitative and quantitative analysis is conducted based on
this information. Label-free quantitative proteomics technology offers simplicity in operation, independence from
expensive labeling reagents, reduced data analysis complexity, and approximately 12-fold improved detection
sensitivity compared to traditional label-free methods.
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Applications of DDA Quantitative Proteomics
Medical Research
Disease Mechanisms, Molecular Diagnosis, Biomarker Development, Drug Targets.
Animal Research
Reproductive Development, Disease Mechanisms, Nutrient Metabolism, Animal Toxicology.
Plant Research
Reproductive Development, Abiotic Stress Response, Disease Resistance, Crop Improvement.
Microbiology
Pathogenic Mechanisms, Drug Resistance, Stress-Related Proteins Screening, Environmental Impact Mechanisms.
Project Experience
Excellent detection stability and identification accuracy. The average number of proteins detected from mouse, human, and plant samples is over 5,000, with up to 6,000+ proteins detected in mouse liver.
Average number of proteins identified
SERVICES
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Project Workflow of DDA Quantitative Proteomics Service
1
Sample Shipment
2
Protein Extraction
3
Trypsin Digestion
4
Data Acquisition
5
Database Search
6
Data Analysis
Sample Requirements of DDA Quantitative Proteomics
| Sample Type | Samples | Recommended Sample Size | Minimum Sample Size |
| Human/Animal Tissue | Normal tissues (heart, liver, spleen, lungs, intestines, kidneys, etc.) | 50mg | 5mg |
| Fatty tissue | 200mg | 100mg | |
| Brain tissue | 50mg | 5mg | |
| Bone | 1g | 200mg | |
| Hair | 500mg | 200mg | |
| Skin | 200mg | 100mg | |
| Plant Tissue | Young tissue (young leaf, seedling, petal, etc.) | 200mg | 100mg |
| Mature tissue (root, stem, fruit, pericarp, etc.) | 1g | 500mg | |
| Pollen | 40mg | 15mg | |
| Liquid Samples | Serum/Plasma (without removing high abundance proteins) | 20μL | 5μL |
| Serum/Plasma (remove high abundance proteins) | 200μL | 100μL | |
| Joint fluid, Lymph fluid | 200μL | 100μL | |
| Aqueous humor, Vitreous body | 300μL | 200μL | |
| Cerebrospinal fluid | 200μL | 100μL | |
| Ascites, Follicular fluid | 100μL | 50μL | |
| Alveolar lavage fluid (BALF) | 1ml | 500μL | |
| Amniotic fluid | 1ml | 500μL | |
| Milk | 20μL | 5μL | |
| Urine | 10mL | 5mL | |
| Saliva (mammals) | 1ml | 500μL | |
| Fermentation broth, Bacterial solution | 10ml | 5ml | |
| Cellular supernatant | 25mL | 10ml | |
| Exosome (sediment) | 25μl | 15μL | |
| Microorganisms | Bacteria | 200mg | 100mg |
| Fungi | 300mg | 150mg | |
| Cells | Primary Cells | 3×10^6 | 1×10^6 |
| Transmissible cells | 2×10^6 | 1×10^6 | |
| Sperm, Platelets | 2×10^7 | 1×10^7 | |
| Protein | Protein | 100μg | 30μg |
Biological duplicates: A minimum of 3 replicates is required; 3-6 replicates for animal samples; 6-10 for clinical samples.
WHAT'S NEXT IN OMICS: THE METABOLOME
Please submit a detailed description of your project. We will provide you with a customized project plan metabolomics services to meet your research requests. You can also send emails directly to support-global@metwarebio.com for inquiries.
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